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21.
ABSTRACT

A protein hydrolysate was prepared from proteins of tuna dark muscle by-product. The hydrolysis conditions (time, temperature, pH, and enzyme concentration) using Alcalase was optimized by response surface methodology (RSM). The regression coefficient close to 1.0, observed during experimental and validation runs, indicated the validity of the model. The hydrolysate produced under the optimum conditions determined by RSM has a low rate of peptide fraction of molecular weight of 4–1 kDa. Meanwhile, the results obtained by hydrolysis under optimal conditions determined by a complementary study (temperature 55°C, time 60 min, 1% enzyme concentration, and pH 8.5) show that the hydrolysate produced has a height rate of the peptide fraction of molecular weight of 4–1 kDa. The amino acid composition of the protein hydrolysate prepared proved to have the potential for application as an ingredient in balanced fish diets and as a source of nitrogen in microbial growth media.  相似文献   
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Temporal variation of rice growth and nitrogen (N) uptake generally follow a sigmoid curve and may respond positively to the N-fertilizer application at critical growth stages. In this study, it was hypothesized that the amount of N-fertilizer applied at critical growth stages possibly follows a geometric pattern such as line, parabola, and sinusoidal to attain maximum yield and nitrogen use efficiency. To test and identify the best pattern, short-term modeling-field testing-long-term modeling strategy was followed. The patterns with the highest simulated yield and nitrogen use efficiency from short-term modeling were tested in the field. Finally, long-term evaluation of N-fertilization patterns was performed using 25 years of historical weather data, resulting in the line pattern with 14% more yield and 25% less NO3? leaching in comparison to the conventional N-Fertilization pattern. Therefore, line pattern may be adopted to enhance the yield and nitrogen use efficiency in rice.  相似文献   
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Proper doses of nitrogenous fertilizer are most important for rice production system because a large part of the nitrogen may be lost if it is not applied judiciously. A study was conducted covering five blocks of Balasore and two blocks of Bhadrak districts. Soil samples were collected randomly, and field visit was conducted during peak vegetative stage of rice. Two approaches have been used in this study for estimating the site-specific nitrogen (N) requirement in the study area. In one approach, geostatisical analysis and kriging was used to develop the soil test–based N recommendation map by which a minimum of 72 kg N ha?1 and maximum of 94 kg N ha?1 were recommended. In a second approach, remote sensing was used and N recommendation map was developed using the moderate-resolution imaging spectroradiometer (MODIS) leaf area index (LAI) and normalized difference vegetation index (NDVI) satellite data, and a minimum requirement of 60 kg N ha?1 and maximum of 120 kg N ha?1 was estimated through this approach.  相似文献   
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Kisspeptins, a family of neuropeptide encoded by the Kiss1 gene, have emerged as crucial regulator of fertility and reproduction by regulating the hypothalamic–pituitary–gonadal axis. The present study was aimed to identify and associate SNPs in the KISS1 gene with reproductive traits in cattle of Indian origin. DNA samples collected from 300 individual cows of three Indian dairy breeds (Gir, Kankrej and Frieswal) of cattle were used in the study. The SNPs of KISS1 gene were identified with PCR-RFLP and sequence analysis using two sets of primer pairs. A total of 5 SNPs were identified in the targeted region of which, two were selected for screening the population and association studies. The analysis revealed that genotypes of rs442633552G>A and rs42022871C>T had a significant association with dry period. The SNP rs42022871C>T also established significant role in milk production traits, and selection of TT-genotyped animals will improve the reproduction and production potential of the animals.  相似文献   
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We hypothesised that digital mapping of various forms of salt‐affected soils using high resolution satellite imagery, supported by field studies, would be an efficient method to classify and map salinity, sodicity or both at paddock level, particularly in areas where salt‐affected patches are small and the effort to map these by field‐based soil survey methods alone would be inordinately time consuming. To test this hypothesis, QuickBird satellite data (pan‐sharpened four band multispectral imagery) was used to map various forms of surface‐expressed salinity in an agricultural area of South Australia. Ground‐truthing was performed by collecting 160 soil samples over the study area of 159 km2. Unsupervised classification of the imagery covering the study area allowed differentiation of severity levels of salt‐affected soils, but these levels did not match those based on measured electrical conductivity (EC) and sodium adsorption ratio (SAR) of the soil samples, primarily because the expression of salinity was strongly influenced by paddock‐level variations in crop type, growth and prior land management. Segmentation of the whole image into 450 paddocks and unsupervised classification using a paddock‐by‐paddock approach resulted in a more accurate discrimination of salinity and sodicity levels that was correlated with EC and SAR. Image‐based classes discriminating severity levels of salt‐affected soils were significantly related with EC but not with SAR. Of the spectral bands, bands 2 (green, 520–600 nm) and 4 (near‐infrared, 760–900 nm) explained the majority of the variation (99 per cent) in the spectral values. Thus, paddock‐by‐paddock classification of QuickBird imagery has the potential to accurately delineate salinity at farm level, which will allow more informed decisions about sustainable agricultural management of soils. Copyright © 2011 John Wiley & Sons, Ltd.  相似文献   
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